In the Wild West of CRISPR genome editing, Cas9 has established itself as a wrangler of animal models that takes out any sequence with an sgRNA bounty on its head. But now there’s a new sheriff in town with marksman aim. CRISPR from Prevotella and Francisella 1 (Cpf1) was brought forth by the pioneering lab […]
EpiGenie Features call out new Methods, provide Insights into emerging trends, or sometimes just share technical tips from the experts in our community.
Navigating the epigenetic landscape is no easy feat. Clear markings and smooth rides through its canals can quickly give way to a hectic hodgepodge of mixed signals and uncertain routes. A great example is bivalent chromatin, which contains marks of both activation and repression. Characterized by Bernstein et al. in 2006, the most studied bivalent […]
Every good captain knows that when your ship is in distress, you send out an SOS. Now, two new papers now leave us wondering if that tactic is borrowed from diseased tissues. Many diseases are characterized by cell death that releases circulating cell-free DNA (cfDNA) into the blood stream, which can now be analyzed in […]
Sequencing technologies have created a dizzying number of opportunities for epigenetic researchers to explore questions that couldn’t be tackled before. Despite the immense power that sequencing delivers, though, the whole process still isn’t quite as simple as pushing a big red button, and watching the groundbreaking discoveries roll in. Even as sequencing becomes faster, easier, […]
RNA-protein interactions are key to understanding human health and disease. Crosslinking-immunoprecipitation (CLIP) and related technologies are powerful tools for characterizing these interactions. Since their introduction in 2008 (Licatalosi et al., 2008), CLIP-based approaches have been applied to prominent RNA research fields such as HIV and cancer. Recently, Sebla Kutluay and colleagues used CLIP to characterize […]