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Technology

Keeping up with the rapidly evolving technology landscape for epigenetics and non-coding RNA research can be daunting. Learn more about the available methods out there and stay on top of the latest developments in our Technology section.

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Gene Editing Future Brightens with paCas9

We knew it was coming.  No, not another Jurassic Park sequel.  No, not another installment of Star Wars.  More inevitable than the revival of the Terminator franchise: we now have photoactivatable CRISPR/Cas9. Yes, as surely as the sun rises, scientists have developed a way to use that light (or at least the blue part) to […]

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Locus-Specific Biochemical Analysis of Genome Functions Using enChIP with CRISPR

Abstract Elucidation of molecular mechanisms of genome functions such as transcription and epigenetic regulation requires identification of components mediating the genome functions. To this end, we recently developed the locus-specific chromatin immunoprecipitation (ChIP) (locus-specific ChIP) technologies to identify molecules interacting with a given genomic region of interest in vivo. Locus-specific ChIP consists of insertional ChIP […]

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None Shall Pass! Genome Editing Stops Heritable Diseases in Their Tracks

The scientific world is still reeling from the news of the first genome editing of human embryos to treat genetic disease, as we have reported previously here on Epigenie. Unfortunately, the paper suggested that this strategy may need huge improvement or may never be applicable. However, another recently published study, from the laboratory of Juan Carlos […]

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Spray Your Way Free of Cystic Fibrosis with a Gene Editing Nasal Spray

With allergy season around the corner, you might be turning to nasal sprays to relieve the symptoms. But now Marie Egan’s lab at Yale University have developed a very different type of nasal spray that helps overcome a disorder much more devastating: Cystic Fibrosis. Earlier attempts to correct the F508del CFTR mutation have been undertaken using CRISPRs in […]

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Catching RNA Polymerases in Action: New NETs for Higher Resolution

For a long time RNA polymerases did not get the credit they actually deserve. They have been viewed as muscle-cars at a drag race: Waiting for a start signal to race along the gene only stopping for the finish line. This view, however, has markedly changed with the development of high-throughput sequencing techniques such as […]