If locus-specific DNA methylation is your game, then you already know that 5-hmC is something that you just can’t ignore. But setting up a round of experiments to figure out the 6th base’s role in your samples is no walk in the park, either. Happily, New England Biolabs has solved that quandary for you with their EpiMark™ 5-hmC and 5-mC Analysis Kit, which profiles both 5-hmC and 5-mC at the same time, using an easy-to-follow protocol.
DNA Methylation Analysis that’s Twice as Nice
NEB’s EpiMark™ Kit doubles your methylation analysis fun by letting you compare both 5-hmC and 5-mC methyl marks simultaneously at any given location. They do this using two restriction enzymes (MspI and HpaII) with differential methylation sensitivity, so you can see how much 5-hmC is around compared to the total methylation.
On Your EpiMark™
The EpiMark™ 5-hmC and 5-mC Analysis Kit comes fully loaded with all the goodies you’ll need to get started and a simple 3-step protocol that’ll get you across the finish line in no time.
- Step 1: Glucosylation treatment;
- Step 2: Restriction enzyme digestion;
- Step 3: Readout with PCR (reagents not included); and
- Step 4 (optional): Show off your stellar finding to your labmates (this step is optional, but highly recommended)
Want more info? Head over to NEB and read more on how you can hit the 5-hmC mark with the EpiMark™ 5-hmC and 5-mC Analysis Kit today.