Chromatin immunoprecipitation (ChIP) remains a powerful method to characterize in vivo binding of DNA hugging proteins like histones and transcription factors and more recently has been used effectively for DNA methylation studies. The ChIP technique has been around since the days of big hair and “Miami Vice,” so we won’t review it here much, but […]
EpiGenie Features call out new Methods, provide Insights into emerging trends, or sometimes just share technical tips from the experts in our community.
It seems pattern recognition and supervised learning techniques are being applied to epigenetics research a lot nowadays. These advanced computational techniques that have proven useful in everything from homeland security to financial modeling are lending a hand to some of the challenges in epigenetics research. Recently we’ve seen them applied to miRNA discovery and target […]
There’s a reason chromosome immunoprecipitation (ChIP) continues to be used in labs all over the world. It works well. In fact, there aren’t too many ways to map minute-by-minute changes at a single promoter, or alternatively, follow a single transcription factor over the entire human genome. ChIP is very versatile and can yield significant insights […]
Since 3’ UTRs were found to contain docking sites for miRNAs, labs with computational muscle around the world have been chipping away the target predication dilemma, but like any game, it’s hard to win when you don’t know the rules. It’s even harder when the rules are being rewritten weekly which is why we have […]
For years, transcriptional regulation took center stage in studies of gene expression, and the nuances of which transcription factor binds to which DNA sequence under which circumstances dominated the dialogue of molecular biologists. In recent years, a supporting actor has threatened to steal the show: enter, post-transcriptional regulation. The idea that messenger RNA utilization could […]