Fast, inexpensive, and accurate, pyrosequencing has become a go-to method for many researchers looking for quantitative methylation data. Designing methylation assays using primers for bisulfite converted DNA can be tricky though, and dealing with assay optimization is no small feat either. Fortunately those worries go up in smoke with QIAGEN’s new PyroMark CpG Assays.
Custom Assay Quality with Off-the-Shelf Ease
With just a few mouse clicks, you can order up CpG island methylation assays from QIAGEN’s database of rigorously designed kits. Here’s what they bring to the party:
- High Coverage: Over 60,000 predesigned assays tailored for highly specific and reliable results
- Optimized: PCR and sequencing primer sets checked against the entire bisulfitome for specificity provided at optimal concentrations for pyrosequencing greatness
- High confidence: 98% generated the correct PCR amplicon, and 92% created successful pyrosequencing results.
- Robustness: Works with highly degraded and precious DNA, like from formalin-fixed, paraffin-embedded (FFPE) tissues.
Also, if you feel like expressing your experimental creativity or there’s not an assay available for your favorite human or mouse locus, you can always build assays yourself using the PyroMark Assay Design Software.
Blazing Fast DNAm Analysis
The beauty of pyrosequencing is that it’s based on sequencing by synthesis, so it can provide quantitative data in sequence context within minutes. And while single-clone Sanger sequencing of 96 samples would take over a week to finish, PyroMark CpG Assays can knock out analyzed results for 96 samples before lunch.
Become a Pyrosequencing Maniac
Now that QIAGEN has taken care of most of the dirty work, all that’s left to do is pump out quality pyrosequencing data. So, get your hands on the bullet-proof PyroMark CpG Assays, and the only thing that’ll be going up in flames will be your competition.
Check out QIAGEN’s site to see how PyroMark CpG Assays can fire up your DNAm experiments.