Researchers that are looking to get started with 5-hmC analysis have plenty of options, but before diving into extensive regional or single-nucleotide 5-hmC profiling, it’s never a bad idea to take a step back and take in the bigger picture.
Global 5-hmC detection is an extremely useful first step in 5-hmC analyses that can save you considerable time and money. Nuclease digestion coupled with liquid chromatography or HPLC is an effective way to detect 5-hmC levels. Sample preparation is relatively straightforward, but you still need to know a thing or two about chromatography and HPLC or get cozy with a colleague who is.
Quest 5-hmC™ DNA ELISA Kits, on the other hand, are about as user-friendly as you can get. But make no mistake; the simplified protocol doesn’t cut any corners when it comes to performance. This system provides:
- Sensitive detection levels of <0.1% 5-hmC genomic content
- Strong data correlation with liquid chromatography and mass spectrometry analysis
- A simple protocol that takes only 3 hours
- An excellent tool for high-throughput profiling
Proven Sensitivity and Specificity
The Quest 5-hmC™ DNA ELISA Kits use one of the most proven laboratory methods, the sandwich ELISA, to rapidly detect 5-hmC with the sensitivity and specificity you’d expect from an assay that’s routinely used in medical diagnostics. The Anti-5-Hydroxymethylcytosine polyclonal antibody included in the kit is the result of screening numerous antibodies for optimal sensitivity and specificity. The system provides sensitive detection levels of <0.1% 5-hmC genomic content and the data correlates strongly with liquid chromatography and mass spectrometry approaches.
Quest 5-hmC™ DNA ELISA Kits data validated by LC-MS/MS-MRM
All’s Well in the Well
Each well is home to a robust detection assay that’s so simple that your PI can get data in a day, if you can manage to drag her away from grant writing. The whole process only takes 5 steps and you’re done in less than three hours. Plus, you don’t need any fancy equipment to get started.
A sandwich-based ELISA with 5-hmC-modified DNA as the filling
Go Global Today
With strong performance delivered in a hassle-free protocol, there’s no reason to put off your dive into 5-hmC analyses. Put the Quest 5-hmC™ DNA ELISA Kits to work today.
