Inside the world’s formalin-fixed, paraffin-embedded (FFPE) tissue repositories exists an enormous store of biological information. This valuable preservation technique allows researchers to perform histological and even genetic analysis years after sample collection.
Although the FFPE process is a valuable preservation process, it presents challenges to researchers seeking to apply epigenetic techniques such as chromatin immunoprecipitation (ChIP) and particularly, ChIP-seq.
In this guide, we’ve accumulated key steps, tips, and tricks to help improve ChIP-seq results when working with FFPE samples including:
An Overview of Unique Challenges of FFPE in ChIP Seq
How does working with FFPE samples differ then regular samples? How does the FFPE process impact the ChIP process and ChIP Sequencing? We answer these questions and others.
A Step by Step Guide on Tackling FFPE Samples with ChIP Seq
From sample recovery to data output, we outline key tips for: Maximizing Chromatin Recovery from FFPE Samples Chromatin Preparation Chromatin Yield and Quality Control Improving Sensitivity of ChIP with FFPE