5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC […]
- DNA Methylation
OneStep qMethyl™ Array
Epigenetic modifications are regarded as fundamental to the regulation of gene expression. DNA methylation is one such modification that plays crucial roles in widespread biological phenomena including the regulation of gene activity, gene imprinting, embryonic development and X-chromosome inactivation in higher organisms. Current methods used to evaluate DNA methylation including bisulfite sequencing, Methylation Specific PCR […]
Quest 5-hmC Detection Kit™
The Quest 5-hmC Detection Kit™ from Zymo Research allows for sequence specific detection of 5-hydroxymethylcytosine within DNA using a simple and efficient reaction setup. Utilizing a robust and highly specific 5-hmC Glucosyltransferase enzyme, 5-hydroxymethylcytosine in DNA is specifically tagged with a glucose moiety yielding a modified base, glucosyl-5-hydroxymethylcytosine. Product Highlights: Method to specifically distinguish 5-hydroxymethylcytosine […]