T4 Phage β-glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, making beta-glucosyl-5-hydroxymethylcytosine.
- 5-hmC Detection
5-hmC Detection
5-Hydroxymethylcytosine, or 5-hmC is a modified form of 5-mC. It has been shown that 5-hmC and the broadly studied 5-mC cannot be distinguished by sodium bisulfite conversion.
EpiMark™ 5-hmC and 5-mC Analysis Kit
New England Biolabs is pleased to introduce the EpiMark™ 5-hmC and 5-mC Analysis Kit, a simple and robust method for the identification and quantitation of 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) within a specific DNA locus. This enzymatic approach utilizes the differential methylation sensitivity of the isoschizomers MspI and HpaII in a simple 3-step protocol (described […]