EpiGenie | Epigenetics and Non-Coding RNA News
Epigenetics & Non-Coding RNA News, Reviews, Interviews and Technology
Bisulfite Conversion allows analysis of DNA methylation patterns. After conversion, amplification of treated DNA is the first step in many methylation analysis protocols.
EpiMark™ Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. An advantage of the [...]
Full DescriptionThe PfuTurbo Cx hotstart DNA polymerase is formulated with a mutant of Pfu DNA polymerase that overcomes uracil stalling completely, allowing the polymerase to read through uracil located in the template strand or incorporated into the extending strand. Since uracil read-through can introduce mutations that adversely impact fidelity, we have further engineered the mutant polymerase [...]
Full DescriptionReduces non-specific PCR product formation from “difficult” templates (e.g., bisulfite-treated DNA). Hot start DNA polymerase for robust product formation. Compatible with real-time, quantitative PCR and suitable for TA-cloning. ZymoTaq’ DNA Polymerase is specifically designed for the amplification of “difficult” DNA templates including: bisulfite-treated DNA for methylation detection, real-time and quantitative PCR that are SYBR Green [...]
Full DescriptionReduces non-specific PCR product formation from “difficult” templates (e.g., bisulfite-treated DNA). Hot start DNA polymerase for robust product formation. Compatible with real-time, quantitative PCR and suitable for TA-cloning. ZymoTaq’ DNA Polymerase is specifically designed for the amplification of “difficult” DNA templates including: bisulfite-treated DNA for methylation detection, real-time and quantitative PCR that are SYBR Green [...]
Full Description
