5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.
Immunogen: This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.
This antibody recognizing 5-formylcytosine has been validated for use in dot blot (1:5,000) and immunofluorescence (IF, 1:2,000), and is stored in rabbit serum containing 30% glycerol and 0.035% sodium azide.
- Antibody Type: Polyclonal
- Host: Rabbit
- Isotype: Serum
- Purification: None
- Appl.: DB, IF
- Reactivity: Mouse, Other (Not Species Specific)