Novus’ ChromataChIP™ Kits are a complete ChIP solution to help you perform chromatin immunoprecipitation efficiently and accurately in your laboratory. We’ve taken all the guesswork out of ChIP by testing multiple component options to make the best kit on the market. Simple to use – ChromataChIP™ is the simplest kit on the market. Our straightforward protocol will allow you to start chromatin immunoprecipitations immediately instead of fumbling throughan overly complicated handbook. Saves money – ChromataChIP™ kits are more cost effective than buying and testing separate components. In addition, all of our components have been individually tested to be the best in class. Since it only takes one component’s failure to ruin an entire assay, our kit eliminates this variable. These critical components include:
- Magnetic Protein A/G beads – With magnetic beads, you save time by performing quicker washes and save money by using less antibody with each IP. Agarose bead users must first pre-clear and block the beads in order to minimize background, which is unnecessary with magnetic beads. Agarose also adds time to every wash because users much separate the beads from washing buffers without pipetting up any of the beads or leaving behind any excess wash, a nearly impossible feat! With magnetic beads, there is no blocking, no centrifugation, and beads completely separate from buffers with the aid of a magnet. In addition, you use 2-5 times less antibody with magnetic beads, making your precious antibody last much longer.
- Positive control antibody and primers – We include a vial of our Epi-plus H3 K4Me3 antibody with every kit. This, along with a human RPL30 primer set, will take the guess work out of ChIP.
- DNA purification columns – These best in class columns consistently give you maximum recovery in under 10 minutes without messy phenol/chloroform cleanup.