EpiSeeker 5-formylcytosine DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify 5-formylcytosine (5-fC) in a microplate-based format. It is suitable for use with DNA isolated from any species (mammals, plants, fungi, bacteria, viruses, etc.) in a variety of forms including, but not limited to, cultured cells, fresh and frozen tissues, paraffin-embedded tissues and body fluid samples.
The function of 5-fC in gene regulation is not yet entirely clear, but it has been shown that 5-fC exhibits replication-dependent dilution during mouse pre-implantation development and could be functional in regulating pre-implantation development as a whole. The detection of 5-fC in various tissues and cells is important because 5-fC could be a marker to indicate active DNAdemethylation. 5-fC can also be directly excised by Thymine DNAglycosylase (TDG) to allow subsequent base excision repair (BER) processing, which converts modified cytosine back to its unmodified state.View EpiSeeker 5-formylcytosine DNA Quantification Kit on Abcam’s website