For complete in vitro methylation of DNA for methylation analysis. Methylation of chromatin DNA for DNA accessibility studies. Inhibition of endonucleases with overlapping GpC sequence recognition. [3H]-labeling of DNA. The GpC Methylase (EC 2.1.1.37)1 from Zymo Research completely methylates all cytosine residues (C5) in double-stranded, nonmethylated and hemi-methylated DNA having the dinucleotide sequence 5?…GpC…3?. The recombinant GpC Methylase (GST fusion) is isolated from an E. coli strain that expresses the methyltransferase gene from a Chlorella virus. The reaction conditions are optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis.
View GpC Methylase -M.CviPI on Zymo Research’s website- GpC Methylase -M.CviPI