Next-Gen Sequencing (NGS) has revolutionized scientific research, but when it comes to epigenetic exploration, scientists have had to make do with sequencing library prep methods from other non-epigenetic applications. The crew at Zymo Research has found a way to adapt sequencers for epigenetic research, without having to completely retrofit the instrument. With the introduction of two new NGS library prep kits, Zymo supplies everything you need, from library prep to purification of your samples, and makes it possible to turn a sequencing machine into one mean Epigenetic Analyzer.
For a Limited time:
Receive a “Blame it on my Epigenetics” T-shirt
with every purchase on either:
Pico Methyl-Seq™ Library Prep Kit
RRHP™ 5-hmC Library Prep Kit
Low Input, Whole Genome Bisulfite Sequencing
Zymo’s first new addition, the Pico Methyl-Seq™ Library Prep Kit, is a post-bisulfite library preparation method for improved Whole Genome Bisulfite Sequencing (WGBS). Pico Methyl-Seq™ has the following interesting features up its sleeve:
- Accommodates ultra-low DNA inputs (down to 10pg), including FFPE samples
- Integrates the award-winning EZ DNA Methylation Lightning™ bisulfite technology
- Simple ligation- and gel-free workflow takes just a few hours
The Pico Methyl-Seq™ Library Kit is more than just a fine-tuned WGBS kit; its unique work flow (fig below) makes it ideal for methylation analysis of precious, limited, and target-enriched samples.
Genome-wide, Strand-specific 5-hmC Profiling
The innovative crew at Zymo also developed a unique method for genome-wide, quantitative analysis of 5-hmC positions. The novel Reduced Representation Hydroxymethylcytosine Profiling (RRHP) technology is at the heart of the new RRHP™ 5-hmC Library Prep Kit, which enables strand-specific mapping of 5-hmC in DNA.
This enrichment-based workflow (fig below) relies on glucosylation of 5-hmC blocking MspI recognition sites This means only DNA fragments containing 5-hmC will be amplified and analyzed at single base resolution using either NGS or arrays.
The RRHP™ 5-hmC Library Prep Kit features a streamlined workflow that is bisulfite-free making it ideally suited for low (≥100 ng) DNA inputs.
Pico and RRHP Join Forces For Simultaneous 5-mC/5-hmC Analysis
Pairing the Pico Methyl-Seq™ and RRHP™ 5-hmC Library Prep Kits together adds even more epigenetic analysis horsepower to your investigations. This dynamic duo can be used to simultaneously assess and differentiate both DNA methylation (5-mC) and hydroxymethylation (5-hmC) marks together, at a genome-wide scale, and at single base resolution. Now that’s a power-packed epigenetics-based NGS offering!
Check out the Zymo Research website for more details on the new NGS Library Prep Kits.