DNA methyltransferases (DNMTs) have been a trending topic in the epigenetics community for years because of their role in cytosine methylation. Studies that set out to pin down the precise function of DNMTs in various disease and developmental states often introduce more questions than answers. To get to the bottom of DNMT function, you need robust functional assays that can consistently determine DNMT activity in a variety of settings.
DNMT Assays for the EpiSeeker in You
Abcam created the EpiSeeker DNMT Assays to meet the needs of epigenetic researchers looking to get the most out of their DNMT explorations. The EpiSeeker line comes in three variations to fit different experimental situations.
- EpiSeeker DNMT Activity Quantification Kits measure total in vivo or in vitro DNMT activity, or inhibition extremely quickly from a 96-well microplate, and are available in colorimetric or fluorometric versions.
- EpiSeeker DNMT Assay Kits determine the amount of DNMT1, DNMT3A or DNMT3B quantitatively from human and mouse fresh tissue or cultured cells without using an electrophoresis and transfer process.
- EpiSeeker Inhibitor Screening Assay Kits selectively screen for DNMT1 or DNMT3B activity, or inhibitors in an ELISA-like format that doesn’t require radioactivity or special equipment.
Off-the-Shelf DNMT Analysis
One thing every EpiSeeker DNMT Assay has in common is that they’re pretty much ready to go from the time you open the box. The kits are formatted in 96 well plates, are based on commonly used lab techniques and come with all the components you need to take on a successful DNMT study. Just bring your sample and you’ll be ready to start.
Find out more details, compare kits and figure out the best EpiSeeker Assay for your lab here at the EpiSeeker DNMT Product Guide.