DNA, it has turned out, isn’t all it was wound up to be. In recent years we learned that the molecule of life, the discovery of the 20th century, did not-could not-by itself explain the huge differences in complexity between a human and a worm. Forced to look elsewhere, scientists turned to RNA; however, methodological […]
EpiGenie Features call out new Methods, provide Insights into emerging trends, or sometimes just share technical tips from the experts in our community.
Proving that great minds think alike, two independent research groups have used similar methods to examine the impact of individual miRNAs on the proteome. In a Nature report by Steven Gygi, David Bartel, and co-workers at Harvard Medical School, the Whitehead Institute for Biomedical Research, and the Massachusetts Institute of Technology, researchers used a quantitative […]
A native of Australia, Susan J. Clark obtained her PhD in 1982 from the University of Adelaide. For the next 8 years, Dr. Clark worked in the biotech industry before returning to basic research where she was instrumental in developing the bisulphite sequencing method for DNA methylation analysis in the early 90s. Dr. Clark is […]
A sensitive new nanotechnology assay might represent a “quantum leap” in DNA methylation detection. The technique, which was developed by Vasudev Bailey and co-workers at The Johns Hopkins University School of Medicine, uses quantum dots-fluorescent nanocrystals of a semiconductor material-to selectivel detect and quantify minute amounts of methylated DNA. As in methylation-specific PCR (MSP), sample […]
It was the best of times, it was the worst of times-one parental allele was transcribing like gangbusters and the other was permanently stalled. Each of us carries a set of genetically distinct chromosomes from mom and dad, and increasing evidence indicates that the two parental genomes also have widespread epigenetic differences. But how can […]