It’s never good to see someone lagging behind the rest, but while stepwise developments to single-cell chromatin immunoprecipitation and subsequent parallel sequencing techniques (ChIP-seq) have led to gradually improved yields and increased sensitivity with reduced numbers of cells since the first report of a single-cell ChIP protocol, we still lack widely-applicable and low-cost methods to […]
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Search Results for: ChIP
Low Cell ChIP-Seq Optimization Module
Maximize retainment of limited ChIP-Seq samples When performing ChIP-Seq on limited samples, maximizing retainment of the already precious sample is key. Active Motif’s Low Cell Optimization Module enables Low Cell ChIP-Seq Kit users to monitor sample retainment as they progress through the protocol. This first-of-its-kind module includes “positive control” samples and antibody, as well as […]
ChIP 101: Beginner’s Guide to ChIP Assays
Become a ChIP Assay Expert! The chromatin immunoprecipitation (ChIP) assay is one of the best ways to study the association of DNA-binding proteins with chromatin and the localization of histone modifications throughout the genome. But ChIP assays take time to master. Our friends at Active Motif have put together this free ChIP 101 eBook that […]
Single Cell ChIP-Seq Seeks Out Cellular Subgroups
When it comes to love, being single has its pros and cons. This is also true for cells, single cells give a lot high-resolution picture, but it’s often very difficult to get enough material. While other single cell methods have flourished with low inputs, studies of the heterogeneity of chromatin states by single cell chromatin […]
ChIP-seq SNFs Out the Epigenomic Impact of SWI/SNF Loss in Paediatric Brain Tumors
Cancer researchers have been SNFing around the role of the SWI/SNF complex for a long time. By SWItching to a ChIP-seq approach, novel details about how biallelic loss of a key gene promotes a cancerous epigenome have been uncovered. SWI/SNF (or BAF) is a chromatin remodeling complex and a key regulator of early development that […]