To methylate, or not to methylate? That is the question. Whether ’tis nobler in the genome to suffer the onslaughts and diversions of oncogenic DNA viruses or, by inactivating, disguise them (from immune surveillance). When researchers used bisulfite sequencing to examine the methylomes of three double-stranded DNA viruses (Human Papilloma Viruses (HPV) 16 and 18, […]
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Search Results for: DNA Methylation
Bisulfite Sequencing the “Other” Nucleic Acid: Methylated RNA
What’s good for the goose is good for the gander, so why shouldn’t bisulfite sequencing be used to study RNA cytosine methylation? Until recently, the harsh reaction conditions required for bisulfite deamination were considered detrimental to RNA stability. However, Matthias Schaefer and colleagues developed an RNA bisulfite sequencing procedure that reproducibly and quantitatively detected cytosine […]
Can Batman Save Whole Methylome Analysis?
With all the hype around Batman-The Dark Knight, we missed one of the most recent heroic efforts to facilitate global methylation studies. The unveiling of a Bayesian tool for methylation analysis (Batman) enables researchers to estimate absolute methylation levels across a range of CpG dense regions using methylated DNA immunoprecipitation (MeDIP) approaches combined with either […]