Being average isn’t all that bad, but it’s far from good. Since its very beginning, chromatin immunoprecipitation (ChIP) has been plagued with the problem of averages: chromatin profiles based on ChIP data are an average of the many thousands of cells required to perform this experiment. This gives us a rough overview of the chromatin […]
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Search Results for: ChIP
Microfluidic ChIP Makes a Few Cells Go a Long Way
Chromatin immunoprecipitation (or ChIP) is a handy technique to study epigenetic profiles, but only if you have enough cells. The main problem with ChIP is that it can be a “greedy” technique that uses large numbers of cells while giving back the bare minimum of DNA as a result. This is problematic for the study of genome-wide […]
ChIPmentation: The Next Fast and Low-Input ChIP-seq Sensation
ChIP-Seq is the bread and butter of histone and transcription factor research. It has seen countless modifications to allow it to perform under all the variables biology can throw at it, but with that specialization has come limitations to generalization. Now the methodological maestros in the lab of Christoph Bock, which brought us single-cell methylome sequencing, […]
Webinar: Key Steps for Validating Antibodies and Optimizing the ChIP Workflow
Abstract: Research in the field of epigenetics has grown at a rapid pace since the discovery of the first histone acetyltransferase enzymes 18 years ago. Since then, significant advances have been made in our understanding of the basic mechanisms of epigenetics (histone acetylation, histone methylation, chromatin remodeling and DNA methylation) and the impact of epigenetic […]
Webinar: Advanced ChIP Normalization Controls
Abstract: Gene expression is regulated by a combination of transcription factor binding and the distribution of epigenetic modifications across regulatory regions. Much of what we know about the epigenome and gene regulation stems from our ability to determine the genome-wide distribution of histone modifications and transcription factors using chromatin immunoprecipitation followed by next generation sequencing […]