Active Motif’s ChIP-exonuclease (ChIP-exo) Kit offers an optimized and streamlined protocol for high-resolution genome-wide mapping of transcription factor binding sites in the human and mouse genomes. The fragmentation step of traditional ChIP protocols can produce oversized DNA fragments that interact with more than just the binding site of interest. ChIP-exo is a modified ChIP-Seq approach that increases the resolution of the binding profile to enable researchers the ability to identify protein-DNA binding sites at a resolution of 20-95 base pairs, compared to traditional ChIP-Seq resolution of 200 bp or more.
The technique refines the ability more precisely define DNA motifs when generating genome-wide protein binding profiles, which provides a significant advantage for studies of mutational or SNP effects on transcription factor binding sites.
- 20-95 bp resolution of protein-DNA binding sites as compared to traditional ChIP-Seq resolution of ≥ 200 bp
- On-bead enzymatic reactions streamline sample processing
- Improved detection of inefficiently-bound transcription factors
- Low background due to exonuclease-mediated degradation of contaminating DNA
- Lower depth of coverage needed due to higher resolution reads and lower background.