Poor DNA methylation enrichment can really leave you in a tight spot when it comes to downstream analysis, so why not turn the tables and put your samples in a bind? Before you begin your next methyl-profiling experiment, make sure you have a solid methylated DNA enrichment kit, like the EpiXplore™ Kit from Clontech, handy to get things going in the right direction.
All the Binding You Want; None that You Don’t
DNA methylation enrichment methods use various MBD proteins to capture methylated DNA, but the EpiXploreTM Kit uses only the binding domain of the MBD2 protein. This set-up means that other components aren’t around to interfere, or bind to unwanted targets and ensures that you’ll get enrichment more specific than a diva’s contract demands. And don’t worry that the chopped down MBD2 lowers sensitivity, Clontech shows that EpiXploreTM can detect as few as three methylated CpGs.
Simple is Good
The EpiXploreTM kit comes with a set of three different elution buffers so that you can fractionate your samples into High, Medium and Low methylation densities, without spending a bunch of time playing with salt concentrations. Add to that a gentle purification process using TALON magnetic beads that keeps your sample intact, and you’ve got a protocol so easy even a caveman (or undergrad assistant) can do it…leaving you that much more time to analyze that treasure trove of data that’s on its way.
Don’t let the stress of your next methylation experiment tie you in knots; have the EpiXploreTM Methyalted DNA Enrichment Kit get you out of a bind.
Find out more details on the EpiXploreTM Methylated DNA Enrichment Kit at the Clontech website.