Abstract: Research in the field of epigenetics has grown at a rapid pace since the discovery of the first histone acetyltransferase enzymes 18 years ago. Since then, significant advances have been made in our understanding of the basic mechanisms of epigenetics (histone acetylation, histone methylation, chromatin remodeling and DNA methylation) and the impact of epigenetic deregulation on cancer, inflammation, metabolism, and neurological diseases. This impact on disease has been underscored by the recent identification of potential oncogenic mutations and losses of epigenetic regulators such as the histone methyltransferase EZH2 and chromatin remodeling protein ARID1A. The chromatin IP (ChIP) assay is a widely used application and has provided a wealth of information regarding the localization and abundance of epigenetic marks and DNA-binding proteins across the genome in many cell types and tissues. In this webinar, Dr. Christopher Fry will present on important factors to consider when performing a ChIP assay, including use of highly validated antibodies, optimized protocols and reagents, and the advantages of using enzyme-based chromatin digestion over sonication-based chromatin fragmentation.
About the Speaker: Christopher Fry is an Associate Director of Product Development at Cell Signaling Technology, where he leads two teams, one that focuses on the development of antibodies against protein and non-protein targets involved in epigenetics, and the other that focuses the development of products for chromatin IP (ChIP).