Webinar: Locus-Specific Biochemical Analysis of Genome Functions Using enChIP with CRISPRJune 17, 2015Abstract Elucidation of molecular mechanisms of genome functions such as transcription and epigenetic regulation requires identification of components mediating the genome functions. To this end, we recently developed the locus-specific chromatin immunoprecipitation (ChIP) (locus-specific ChIP) technologies to identify molecules interacting with a given genomic region of interest in vivo. Locus-specific ChIP consists of insertional ChIP […]
Webinar: An Engineered Synergistic CRISPR/Cas9 Activator Complex for Genome-wide Transcriptional ControlMarch 4, 2015Presenter: Silvana Konermann, Graduate Student in Dr Feng Zhang’s Lab at Massachusetts Institute of Technology Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. The ease and scalability of the CRISPR-Cas9 system potentially enables systematic, genome-scale perturbation, but the magnitude of transcriptional up-regulation achieved by the first generation of Cas9 transcriptional […]
Webinar: Using CRISPR/Cas9 Technology for Editing Genes in Haematopoietic Cells and MiceJuly 29, 2014Presented by: Dr Marco Herold, Laboratory Head, Molecular Genetics of Cancer Division, The Walter & Eliza Hall Institute of Medical Research Abstract: The CRISPR/Cas9 technology provides an easy and rapid way to edit genes in vitro and in vivo. Initial experimental strategies utilized a transient transfection approach to modulate genes in vitro, but this proved […]
An Introduction to Epigenetic Editing with Dr. Marianne RotsJune 23, 2014Dr. Marianne Rots, Professor of Molecular Epigenetics and Head of Epigenetic Editing Laboratory at University of Groningen, Netherlands provides an informative overview of the current state of epigenetic editing approaches. Intro to Epigenetic Editing Epigenetic editing exploits the two main characteristics of epigenetics. On the one hand, epigenetic marks are heritable. This means the epigenetic […]