ChIP-Seq has transformed the field of epigenetics, providing unprecedented resolution and coverage for surveying protein- DNA interactions. Powerful, high-throughput sequencing is credited with most of the benefits, but there’s a lot more that goes into ChIP-Seq than sequencing. Chromatin shearing is one of the first critical steps in the ChIP-Seq workflow. Depending on your approach, […]
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Features
EpiGenie Features call out new Methods, provide Insights into emerging trends, or sometimes just share technical tips from the experts in our community.
MeCP2 on the Brain: Repressing Transcription of Long Highly Methylated Genes
It seems that everyone has MeCP2 on the brain – as evidenced by its starring role in two new Molecular Cell papers – but it’s in your brain too! The MeCP2 protein is essential for neuronal function and consistent with this, loss-of-function mutations in MeCP2 cause the neurodevelopmental disorder Rett syndrome. Unfortunately, because MeCP2 acts […]
A Perfect Harmony: Orchestrating Joint Chromatin Conformation and Methylome Mapping in Single Cells
If a better understanding of gene regulation is music to your ears, then tune in for the latest developments in single-cell epigenomics. It is well established that both DNA methylation and higher-order chromatin structure play crucial roles in development, differentiation and disease, and there are readily available technologies for both (whole-genome bisulfite sequencing and chromatin […]
Webinar: The Epigenetics of the Exposome and Aging – Paul G. Shiels
Don’t miss this great webinar detailing some of the latest discoveries about the role of epigenetics in aging. Aging results in a gradual and progressive loss of physiological functions and physical abilities over time. Overcoming the growing burden of age-related issues is one of the greatest challenges facing modern society. These issues are leading to […]
The Tn5 Transposase Takes Single-cell ChIP-Seq to the Next Level
It’s never good to see someone lagging behind the rest, but while stepwise developments to single-cell chromatin immunoprecipitation and subsequent parallel sequencing techniques (ChIP-seq) have led to gradually improved yields and increased sensitivity with reduced numbers of cells since the first report of a single-cell ChIP protocol, we still lack widely-applicable and low-cost methods to […]