eBook: DNA Methylation 2024May 15, 2024DNA methylation, whether in cells or in a cell-free environment, is one of the most powerful and most studied epigenetic regulators of gene expression and genomic organization. This is true for nearly all organisms, leading to the emergence of numerous methods for mapping 5-mC and 5-hmC and other modification states. There are many approaches for […]
eBook: New & Novel Methods for Epigenetic AnalysisFebruary 7, 2024Epigenetics is not new, but methods used for analysis certainly can be! In this eBook, we take a look at, and present in a simple format, some of the hottest new methods for researchers looking at DNA Methylation, RNA modifications, Chromatin and Gene Regulation. Topics Include: Differential Methylation Data Are Quick, Easy, and Inexpensive with […]
uCoTarget Is a Single-Cell Multiomics Method for Multiple Histone ModificationsJanuary 17, 2024Everyone loves a bargain, and a new method called CoTarget really delivers. It’s a method that delivers single-cell multiomics data on multiple histone modifications and transcription factors, as well as the transcriptome. It can easily scale up to analyze a million single cells, and unlike similar methods, it doesn’t require microfluidic devices or special transposon […]
The Brain Cell Census: A Deeper Epigenomic Understanding of the Mammalian MainframeNovember 13, 2023If you have epigenetics on the brain like us, concentrate your mammalian mainframe on the recently published marvels on the brain’s epigenome from the NIH’s Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Initiative – Cell Census Network (BICCN). Recent work brought together under the guise of the Brain Cell Census now reports on the examination […]
Kacme: A New Histone Modification Couples Methylation and Acetylation to Mark Active TranscriptionOctober 23, 2023Some couples seem unlikely, yet somehow, they work (a recent pop star-football player pair comes to mind). Now, an even more surprising couple—a methyl group and an acetyl group—hanging out together on the very same lysine residues on histone H4s has just been spotted across species and appears to be involved in active transcription. Mind. […]
eBook: RNA & TranscriptomicsOctober 13, 2023Analysis of the working of our RNA, from transcription and splicing to translation, began in a simpler time – when we talked about “RNA” and hadn’t yet recognized the whole range of RNA species, including miRNAs, ncRNAs, and more. These days, we are learning as much about the epigenetic modification of RNA and the role […]
Epigenetic Inheritance During DNA Replication: A State Lying Somewhere Between Symmetry and InstabilityOctober 2, 2023Emerging research has provided evidence for the critical importance of the inheritance of epigenetic states, whether that be DNA methylation, histone modifications, or even non-coding RNAs. Now, three recent studies focusing on the mechanism involved in passing down this information from parental to daughter cells while they replicate their DNA and divide in vitro provide […]
Just Add HiDRO to Figure Out How Genomes Fold in Single CellsSeptember 14, 2023Water hydrates and is essential for life—for plants, humans, and even fish. So, it’s fitting that a new technique called HiDRO takes high-throughput FISH to the next level to identify factors that help genomes fold in 3D at high throughput in single cells. Cohesin helps DNA form loops, which can then form topologically associated domains (TADs). […]
An Evolutionary and Epigenetic Tour-de-force Links DNA Methylation and Mammalian AgingSeptember 11, 2023Have you climbed Mount Everest? Recorded a critically acclaimed album?? Written the great American novel??? These life-affirming feats of body and mind pale into insignificance when compared to the evolutionary and epigenetic tour-de-force reported in two recent papers based on mammalian DNA methylation datasets of colossal size and importance. An expeditionary force led by Steve […]
eBook: Chromatin & Gene ExpressionJune 14, 2023Gene expression is regulated by a range of factors, but ultimately begins with chromatin – it’s structure, configuration, activation, and “openness”. All of these factors can be dynamically regulated by epigenetics. In this eBook, we bring to your attention some of the most interesting articles in chromatin biology and gene expression, from practical methods to […]