Methyl-binding proteins are rapidly becoming a preferred solution for researchers looking to boost the sensitivity of their DNA methylation analysis. Current approaches that use antibodies for DNA methylation enrichment have been useful to many, but alternative methods that use methylation binding proteins like the MBD2 in Invitrogen’s MethylMiner™ Kit grip methylated DNA tighter than skinny jeans on a rockstar. This enhanced binding provides researchers the opportunity to glimpse into the regions of differential methylation that elude other approaches. How does MethylMiner™ pull it off? Here are a couple of reasons:
It binds double stranded DNA
MethylMiner™ binds DNA in its native, double-stranded state so you don’t need to worry about denaturing your sample. More importantly, you don’t have to worry about single-stranded DNA re-annealing which can dampen the enrichment process, reducing sensitivity.
It doesn’t require proteinase K digestions and clean up
Unlike many antibody-based approaches, the MethylMiner™ protocol ditches proteinase K digestions and the related clean up steps. This helps you hold on to your precious sample by eliminating another area where some can be lost.
Just Bead It: Dynabeads® Round Off the Purification Process
Bead-based purification can really take the sting out of tedious purification processes that can introduce sample loss, not to mention increase your protocol length. MethylMiner™ uses the superparamagnetic Dynabeads®, making the whole enrichment process smoother than a Barry White song.
Adaptability: Less Work and More Ligation
Many researchers today want to sequence their enriched methylation samples. MethylMiner™ makes library construction for sequencing a breeze as the enriched samples remain double-stranded throughout the whole enrichment process and are primed to have sequencing adaptors tacked on their ends.
With MethylMiner™, fewer steps, less time, and minimal manipulation help you to hold on to more of your sample and improve the sensitivity of your DNA methylation enrichment. Sound good? Give it a try!
Get more information on the MethylMiner™ Methylated DNA Enrichment Kit at the Invitrogen website.