Guide: Optimizing ChIP Seq for FFPE Samples
Inside the world’s formalin-fixed, paraffin-embedded (FFPE) tissue repositories exists an enormous store of biological information. This valuable preservation technique allows researchers to perform histological and even genetic analysis years after sample collection.
Although the FFPE process is a valuable preservation process, it presents challenges to researchers seeking to apply epigenetic techniques such as chromatin immunoprecipitation (ChIP) and particularly, ChIP-seq.
In this guide, we’ve accumulated key steps, tips, and tricks to help improve ChIP-seq results when working with FFPE samples including:
- An Overview of Unique Challenges of FFPE in ChIP Seq
- A Step by Step Guide on Tackling FFPE Samples with ChIP Seq
Optimizing ChIP Seq for FFPE Samples
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