PRODUCT OVERVIEW Twenty aliquots of 2 mM S-adenosylmethionine (25 μl each) for use in methyltransferase reactions. At neutral pH, S-adenosylmethionine is easily oxidized. This pH-stabilized formulation has been evaluated by CH3 BioSystems to produce methylated product in standard methyltransferase reactions when stored properly. PURIFICATION NA FORMULATION Proprietary RECOMMENDED USAGE Dilute 1:20 for 100 μM working […]

PRODUCT OVERVIEW Arginine-Rich Peptide is a synthetic 1.6 kilodalton peptide that can be used to detect arginine methyltransferase activity in cell/tissue extracts and other biological fluids. Detection of the presence of methylarginine can be performed with anti-mRG methylarginine-specific antibody or by film based detection methods that utilize a radioactive methyl donor in the assay reaction. […]

PRODUCT OVERVIEW PRMT7 is a unique recombinant type III protein arginine methyltransferase expressed as a fusion protein in bacteria. As supplied, the enzyme is extremely active and capable of mono-methylation of arginine acceptor sites in peptides and proteins under appropriate incubation conditions. PURIFICATION Glutathione Sepharose chromatography. FORMULATION Tris Buffer pH 8. RECOMMENDED USAGE In vitro […]

PRODUCT OVERVIEW PRMT1 is a recombinant type I protein arginine methyltransferase expressed as a fusion protein in bacteria. As supplied, the enzyme is active and capable of methylating arginine acceptor sites in peptides and proteins under appropriate incubation conditions. PURIFICATION Glutathione Sepharose chromatography. FORMULATION Tris Buffer pH 8. RECOMMENDED USAGE In vitro methylation of proteins […]

PURIFICATION Protein A Sepharose chromatography. FORMULATION Tris Buffer pH 8 + 0.02% sodium azide. RECOMMENDED USAGE Western Blotting; Immunoprecipitation; Immunocytology. TESTING All lots of ANTI-mRG exhibit avid reactivity with 10 ng of a methylarginine peptide by western blotting, but no detectable reactivity with 100 ng of an arginine peptide, the sequence of which is identical […]