Linker-1 is the original modban sequence employed by Lau and Bartel in 2001 and contains a Ban-I restriction site. Linker-2 contains Ava-I and Sty-I restriction sites. Linker-3 contains EcoRI and Msp-I restriction sites and was adapted from Pfeffer and Tuschl. All three linkers are modified with a 3′-terminal dideoxy-C (ddC) base to prevent self ligation. […]