Henrietta Lacks and Chinese hamster ovaries have all spawned cell lines that are cornerstones of biological research, but sometimes the chromatin immunoprecipitation (ChIP) answers you’re looking for can’t be found in the flasks of cultured cells.
Studying chromatin and histone modifications in animal materials provides a snapshot of the actual, region specific, physiological conditions of a sample. Of course, it also comes with complications compared to using cell-lines, like the added chore of upfront sample prep and the complexities of a potentially mixed cell population.
EMD Millipore makes this whole process as painless as possible with their Magna ChIP™ G Tissue Kit that’ll have you cranking out tissue sample ChIP analyses with ease.
Smoothing Out the Tissue ChIP Process
Millipore leveraged the considerable talents of their R&D crew to find reliable and optimized reagents in order to extract high quality chromatin from stubborn tissues.
To begin with, they incorporated a 1mm micro-dissection punch which let’s you isolate tissues from precise sites within a sample. Next the team re-worked the whole chromatin isolation process, including the development of a proprietary tissue stabilization solution, from the ground up to create a optimized protocol just for use with tissues. Their combination of tweaks made ChIP performance with tissues go through the roof.
The resulting kit and protocol has been successfully used to tackle micro-dissected samples and tissue biopsies from brain, heart, lung, liver, and kidney, so you can rest easy knowing that most of the optimization has been done for you. So go ahead and break out all of those tissue samples.
- This graphic either explains how the Magna ChIP™ process works, OR you’re playing Centipede. Either way, it’s good times.
A Magna ChIP™ Kit for Any Occasion
ChIP experiments on tissue samples not your cup of tea? It’s OK. Whether you need a standard Protein A or G Kit, want it with or without controls, or need kits that will set you up for full genome wide analysis like ChIP-seq or ChIP-chip, EMD Millipore has more flavors than Baskin Robbins. See the full array of Magna ChIP™ Kits here.
You can learn more about the Magna ChIP™ G Tissue Kit at the EMD Millipore website.