If you’re kicking off an expedition to explore the great hydroxymethylated unknown, make sure you’re geared up with the right equipment. Until recently, 5-hmC detection methods could only give you a general idea of the hydroxymethylation profile of your sample, but the new Quest 5-hmC Detection Kit™ let’s you see exactly where those 5-hmC marks have been hiding all this time.
Site-Specific 5-hmC Detection
The secret is in the 5-hmC Glucosyltransferase that tacks glucose onto whatever 5-hmCs it sees, protecting them from glucosyl 5-hydroxymethylcytosine sensitive restriction enzymes (GSREs) that would normally chop 5-hmC and 5-mC to bits. Armed with these differential digestion fragments, 5-hmC will have no where to hide.
Quest and Quest Lite. Same great detection, but less filling.
Simplicity from Setup to Sequencing
The whole single tube reaction is a breeze to set up, especially for anyone who’s played around with in-vitro enzyme reactions before. And, best of all, the cleaned up product is ready to go straight into a boat-load of your favorite analysis methods from the old stand-by, PCR, to the new fangled NextGen sequencers.
Quest –Lite: Same Peformance, More Flexibility
The Quest 5-hmC Detection Kit™ comes complete with MspI enzyme and everything else you need to rock-n-roll straight from the box. But for those researchers who like to have options, there’s also the Quest 5-hmC Detection Kit™ – Lite. The Lite version doesn’t have a GSRE enzyme in it for those researchers who may be keen on using another enzyme…or even an enzyme cocktail with different recognition sites for some ultra-thorough analysis.
Before your next quest to understand the mysteries of the 6th base, find out more about the Quest 5-hmC Detection Kit™ at the Zymo Research website.