Imaging of single cell nuclei is now providing evidence for the stochastic properties of genome function organization that may contribute to cell-type specific gene expression and to the dynamic responses that occur during differentiation and adaptation to the environment. In this workshop we will bring together leaders in the field of nuclear structure and function, with a focus on imaging and modelling the behaviour of regulatory nuclear proteins, the genome, and the organisation of replication and transcription in single cells. A broad range of approaches will be covered from fluorescence microscopy of fixed samples combined with advanced image analyses, to in vivo imaging of RNA and proteins, to super-resolution microscopy and modelling of polymers in the presence of chromatin binders.
- Imaging the Cell Nucleus Workshop