Each EpiMark Bisulfite Conversion Kit contains sufficient reagents to perform the bisulfite conversion reaction of 48 samples. For optimal results, 50 ng–2 μg DNA is recommended.
Kit Components:
- Sodium metabisulfite (each aliquot is sufficient for 8 reactions)
- Solubilization Buffer 2 x 1 ml
- Desulphonation Reaction Buffer (concentrate) 3 ml
- EpiMark spin columns with 2 ml collection tubes 50
- Binding Buffer 30 ml
- Wash Buffer (concentrate) 2 x 10 ml
- Elution Buffer 4 ml
Required Materials Not Included:
- Heat block or water bath (requiring temperatures of 65°C and 92°C)
- 96–100% molecular biology grade ethanol
- PCR Thermal Cycler
- 0.2 ml strip tubes and caps
- 1.5 ml reaction tubes
- Nuclease-free Water
- Pipettors and pipette tips (for minimization of cross contamination, use aerosol barrier tips)