New Products

CUT&Tag-IT™ Assay Kit Overview Cleavage Under Targets and Tagmentation (CUT&Tag) is a method to investigate genomic localization of histone modifications and some transcription factors that reveals interactions between proteins and DNA or identifies DNA binding sites for proteins of interest. Unlike MNase-Seq or ATAC-Seq methods that target open chromatin and are therefore dependent on chromatin […]

PIXUL™ Multi-Sample Sonicator Overview Next-generation sequencing applications, such as ChIP-Seq, RNA-Seq, RIP-Seq, exome sequencing, and whole genome sequencing require precise sample fragmentation (200-600 bp) for compatibility with short read sequencing platforms. Shearing by physical sonication, as opposed to enzymatic methods, is preferred for its unbiased nature and ability to produce a relatively narrow fragmentation profile. […]

Long noncoding RNAs (lncRNAs) have been shown to play important roles in the regulation of both transcription and chromatin organization. But, the promoters of lncRNA genes appear to be regulated differently than those of protein-coding genes. The LightSwitch Luciferase Assay System makes it fast & easy to study lncRNA promoter regulation using the same materials […]

The Tag-ChIP-IT® Kit offers a method to perform chromatin immunoprecipitation (ChIP) on transcription factor sequence variants, mutations and truncations without the need for protein-specific antibodies. Simply create an expression construct containing the protein of interest in-frame with a C-terminal AM-tag. Active Motif’s unique AM-tag sequence is specifically designed to work in ChIP. It has minimal […]

Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is […]

Whether you are performing drug discovery screening, analyzing enzyme kinetics or monitoring changes in histone modifications, substrate selection is critical. Nucleosomes offer the advantage of providing a more biologically relevant substrate when compared to histone proteins alone or synthetic peptides. To best mimic cellular biology with your in vitro assay, use Active Motif’s Recombinant Nucleosomes. […]

Active Motif’s new ChIP-Bisulfite-Sequencing (ChIP-Bis-Seq) Kit offers a highly optimized and user-friendly method to directly interrogate both the chromatin and DNA methylation profile on the same sample within a single experiment. The kit combines a chromatin immunoprecipitation (ChIP) procedure for target-specific enrichment of protein-bound or modified chromatin with bisulfite conversion and sequencing (Bis-Seq) to provide […]

Abcam’s High-Sensitivity ChIP Kit (ab185913) is a complete set of optimized reagents to carry out a successful chromatin immunoprecipitation procedure in a high throughput format starting from mammalian cells or tissues. The highly specific and sensitive kit is suitable for selective enrichment of a chromatin fraction containing specific DNA sequences using various mammalian cell/tissues. The […]

Global DNA Methylation Assay to quantify genome-wide 5-mC levels in human DNA Simple ELISA assay in a flexible strip-well microplate format Detects as low as 0.5% methylation Detects methylation from as little as 10 ng DNA/well LINE-1 probe used for DNA capture for better specificity Biologically relevant standards provided for improved quantification Active Motif’s Global […]

Histone purification system for high throughput applications Active Motif’s Histone Purification Microplate Kit enables purification of core histones (H2A, H2B, H3 and H4) using very small amounts (as little as 0.9 x 105 cells) of starting material, and its microplate spin column format enables elution into a 96-well plate to easily transition into downstream high-throughput […]

Perform ChIP on difficult-to-lyse PBMCs: Active Motif’s ChIP-IT® PBMC Kit overcomes the challenges of generating quality chromatin for use in ChIP from peripheral blood mononuclear cells (PBMCs). PBMCs, including lymphocytes (T cells, B cells and NK cells), monocytes and dendritic cells, constitute a critical component of the peripheral immune system and are often used in […]

Perform ChIP from FFPE samples Formalin-fixed paraffin-embedded (FFPE) clinical samples are a valuable resource for retrospective research. However, working with FFPE samples presents a challenge for techniques such as ChIP. Active Motif has leveraged its ChIP expertise to develop the first commercially available kits to enable ChIP from FFPE tissue for use in Next Generation […]

The HDAC-Glo™ Class IIa Assays are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) Class IIa from cells, extracts or purified enzyme sources. The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a coupled […]

The HDAC-Glo™ 2 Assays are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) Class I enzyme 2 from cells, extracts or purified enzyme sources. The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a […]

The Zymo-Spin™ ChIP Kit from Zymo Research provides a streamlined ChIP procedure for investigating protein-DNA interactions that have been “fixed” in their natural state and can be used to effectively identify binding sites for transcription factors, co-factors, and other DNA regulatory proteins. Features: Unique workflow features a micro-elution (≥6 µl) spin column for purification of […]

The EZ RNA Methylation™ Kit features rapid and reliable bisulfite treatment and conversion of cytosines in RNA for methylation analysis. The kit streamlines the three-step process for complete conversion of cytosine in into uracil. RNA denaturation and bisulfite conversion processes are combined into a single step. No buffer preparation is necessary. The RNA Conversion Reagent […]

Don’t let some big corporation dictate your ChIP protocol! For researchers interested in streamlining their own workflow, Active Motif offers Protein G Agarose Columns. These are ready-to-use ChIP columns that contain the same high affinity protein G agarose beads included in the ChIP-IT® High Sensitivity Kit. The beads have been specifically engineered to eliminate non-specific […]

EpiSeeker 5-formylcytosine DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify 5-formylcytosine (5-fC) in a microplate-based format. It is suitable for use with DNA isolated from any species (mammals, plants, fungi, bacteria, viruses, etc.) in a variety of forms including, but not limited to, cultured cells, fresh and frozen […]

The LightSwitch™ Luciferase Assay System includes over 30,000 regulatory elements from the human genome that have been cloned into LightSwitch reporter vectors. With over 18,000 human promoters and 12,000 3´UTRs available as transfection-ready LightSwitch luciferase reporter vectors, you can perform reporter assay experiments immediately. The LightSwitch System makes it easy for you to measure the […]

The MethylEdge™ Bisulfite Conversion System provides a rapid, efficient method to perform bisulfite conversion with minimal DNA fragmentation in less than two hours. The rapid protocol and complete conversion mean that you can produce completely converted DNA ready for your choice downstream assays with minimal preparation and hands-on time. Coupled with purification chemistries and flexible, robust amplification […]

The combination of ChIP with genome-wide analysis using Next-Generation sequencing (ChIP-Seq) is a powerful approach that can provide insights into gene regulation by identifying global binding sites for proteins of interest. Active Motif’s ChIP-IT® ChIP-Seq Kit provides proven reagents, streamlined protocols and validation controls to give you confidence in successful ChIP-Seq using the Illumina®sequencing platforms. The […]

The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification. This kit includes two different enzymes for nucleosomal DNA preparation: Atlantis dsDNase and micrococcal nuclease. Atlantis dsDNase is a double-strand DNA specific endonuclease […]

SureSelectXT Human Methyl-Seq is the first comprehensive target enrichment system to enable researchers to focus on the regions where methylation is known to impact gene regulation: CpG islands, CpG island shores, undermethylated regions, promoters, and differentially methylated regions (DMRs). SureSelectXT Human Methyl-Seq Uniquely delivers more information than methylation microarrays by detecting individual CpGs Increases throughput […]

The ChIP-IT® High Sensitivity (HS) Kit is designed to give superior performance when using antibodies against low abundance transcription factors, antibodies with sub-optimal binding affinities or when starting with a limited number of cells. Higher signal and lower non-specific DNA pulldown is achieved through the incorporation of specialized protein G agarose beads and optimized fixation, […]

NOMe-Seq is a first of its kind kit to simultaneously analyze nucleosome occupancy and CpG methylation status on the same DNA strand. NOMe-Seq (Nucleosome Occupancy and Methylome sequencing) is a published method that was developed by the Peter Jones lab at USC and has been validated for gene-specific analysis using both traditional cloning and Sanger […]

The ability to efficiently detect and quantify DNA methylation (i.e., 5-methylcytosine) has become essential for epigenetic-based research.  To date, a number of methods have been developed for this purpose including high-performance capillary electrophoresis, bisulfite sequencing, and methylated DNA immunoprecipitation. The 5-mC DNA ELISA Kit is a convenient and powerful tool that allows the researcher to […]

The Quest 5-hmC™ DNA ELISA Kit is both sensitive and specific and can be used to accurately detect 5-hmC DNA in a variety of samples. The kit is compatible with a wide range of input DNA including intact vertebrate, plant, and microbial genomic DNA, as well as enzyme-digested and mechanically sheared fragments. The Control DNA […]

Specific glycosylation of 5-hydroxymethylcytosine (5-hmC) 5-hmC Glucosyltransferase catalyzes the transfer of a glucose moiety from uridine diphosphoglucose (UDPG) to existing 5-hmC in DNA to form glucosyl-5-hydroxymethylcytosine (glucosyl-5-hmC). 5-hmC Glucosyltransferase does not modify cytosines or methylated cytosines (5-mC). Glucosylation of 5-hmC can be used for sequence-specific, locus-specific, or global quantification of 5-hmC. Validation: 5-hmC Glucosyltransferase was […]

CpGenome™ Human Methylated & Non-methylated DNA Standards are purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. The CpGenome™ Human Methylated DNA Standard has been methylated enzymatically at all CpG dinucleotides by M. SssI methyltransferase 2. These standards are […]

CpGenome™ 5-mC and 5-hmC DNA Standard Set contains three linear, double-stranded DNA (897 bp) with the same sequence, but each contains 100 % 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines. This set can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications. Materials Provided: Three vials, each containing 2 µg (40 µL) […]

Benchtop shearing for NGS and ChIP M220 Focused-ultrasonicator is designed for Next-Gen Sequencing and ChIP applications. The compact size and ease-of-use of the M220 Focused-ultrasonicator make it the ideal Chromatin shearing solution for any lab.  Compatibility with the truChIP™ Low Cell Chromatin Shearing kits provides sensitive ChIP assays with fewer than 1 million cells. Integrated […]

Novus’ ChromataChIP™ Kits are a complete ChIP solution to help you perform chromatin immunoprecipitation efficiently and accurately in your laboratory. We’ve taken all the guesswork out of ChIP by testing multiple component options to make the best kit on the market. Simple to use – ChromataChIP™ is the simplest kit on the market. Our straightforward […]

Epi-Plus™ antibodies are the most well validated antibodies available for epigenetics research. All Epi-Plus™ antibodies are multi-assay validated, including testing by dot blots using modified peptide arrays, western blots, and chromatin immunoprecipitation (ChIP). Novus Biologicals and 21st Century Biochemicals have collaborated to produce this superior line of Epi-Plus™ antibodies.

The AbSurance™ Complete Core Histone Antibody Specificity Array is comprised of 89 high quality modified and unmodified histone peptides ( >95 % purity) spotted on Immobilon® P PVDF membranes in a 96-well format. One membrane contains 46 histone H3 peptides (8 acetylated, 27 methylated, 5 phosphorylated, and 6 unmodified). The other membrane contains 43 histone […]

The EZ DNA Methylation-Lightning™ Kit features rapid and reliable bisulfite treatment and conversion of DNA for methylation analysis. Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is necessary, simply add this unique reagent to a DNA sample, wait about an hour, and let the reaction proceed to completion. DNA denaturation […]

Active Motif’s MethylCollector™ Ultra offers a fast magnetic assay capable of efficiently isolating methylated CpG islands from fragmented genomic DNA. MethylCollector Ultra is based on the Methylated CpG Island Recovery Assay (MIRA) which uses a MBD2b/MBD3L1 protein complex for improved enrichment of CpG dinucleotides. Enriched DNA can be used in many downstream applications, such as […]

The traditional ChIP methods, based on agarose or magnetic beads, involve many steps of separation, pipetting and re-suspension. These sequences can either be done by magnetism or centrifugation, which are repetitive and prone to errors. Chromatrap® is a new technology that offers a quicker, easier and more efficient way of undertaking chromatin immunoprecipitation assays. These kits […]

The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument. Each kit includes a complete set of validated, quality controlled reagents, positive and negative […]

The Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument.Each kit includes a complete set of validated, quality controlled reagents, plus a detailed protocol. […]

The Hydroxymethyl Collector™ Kit is designed to detect and capture DNA fragments containing 5-hydroxymethylcytosine (5-hmC) DNA methylation. The Hydroxymethyl Collector kit utilizes a β-glucosyltransferase enzyme to transfer a modified glucose moiety to 5-hydroxymethylcytosine residues in double-stranded DNA. This modified glucose is then used to chemically attach a biotin conjugate for capture and enrichment with streptavidin […]

5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC […]

5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC […]

Epigenetic modifications are regarded as fundamental to the regulation of gene expression. DNA methylation is one such modification that plays crucial roles in widespread biological phenomena including the regulation of gene activity, gene imprinting, embryonic development and X-chromosome inactivation in higher organisms. Current methods used to evaluate DNA methylation including bisulfite sequencing, Methylation Specific PCR […]

The SIRTainty™ Class III HDAC Assay utilizes a novel patent pending technology for the sensitive detection of all known sirtuin family members. This easy to perform assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a substrate providing a direct assessment of the activity of class III HDAC […]

The Quest 5-hmC Detection Kit™ from Zymo Research allows for sequence specific detection of 5-hydroxymethylcytosine within DNA using a simple and efficient reaction setup. Utilizing a robust and highly specific 5-hmC Glucosyltransferase enzyme, 5-hydroxymethylcytosine in DNA is specifically tagged with a glucose moiety yielding a modified base, glucosyl-5-hydroxymethylcytosine. Product Highlights: Method to specifically distinguish 5-hydroxymethylcytosine […]

Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome […]

Perform automated ChIP (Chromatin Immunoprecipitation[application ChIP]) from as low as 1000 cells. Ideal for Stem cells, embryos and biopsies. The Diagenode’s Auto ChIP kit is designed to perform automated immunoprecipitation of Chromatin using the SX-8G IP-StarTM Automated System enabling highly reproducible results and allowing for high throughput. Use from 1,000 – 100,000 cells per IP […]

Perform Automated MeDIP (Methylated DNA Immunoprecipitation) to estimate DNA methylation status of your sample using the 5-methylcytidine antibody. Diagenode’s Auto MeDIP kit has been optimized on the SX-8G IP-StarTM Automated System enabling highly reproducible results and allowing for high throughput assays. Generate highly consistent results with internal controls in 24h Minimize error with many reagents […]

The Bioruptor® uses a unique system to uniformly process multiple samples in sealed tubes of 0.5ml to 50ml capacity. An excellent device for shearing chromatin or DNA, cell lysis and many other applications: Chromatin Shearing DNA Shearing cell Lysis Disruption Cleaning Disaggregation Extraction Dissolution Emulsification Homogenization Comminution Sonochemistry Dispersion Mixing Synthesis Catalysis Degassing Microencapsulation Hydrolyzation […]

CUT&Tag-IT™ Assay Kit Overview Cleavage Under Targets and Tagmentation (CUT&Tag) is a method to investigate genomic localization of histone modifications and some transcription factors that reveals interactions between proteins and DNA or identifies DNA binding sites for proteins of interest. Unlike MNase-Seq or ATAC-Seq methods that target open chromatin and are therefore dependent on chromatin […]

Analyze open chromatin regions at genome-wide scale. ATAC-Seq is a rapid assay that allows analysis of epigenetic profiles across the genome by identification of regions that have open or accessible chromatin states. Because of the assay’s speed, simplicity, and applicability to a wide range of sample types, ATAC-Seq has become a commonly-used epigenetic assay, and […]

Maximize retainment of limited ChIP-Seq samples When performing ChIP-Seq on limited samples, maximizing retainment of the already precious sample is key. Active Motif’s Low Cell Optimization Module enables Low Cell ChIP-Seq Kit users to monitor sample retainment as they progress through the protocol. This first-of-its-kind module includes “positive control” samples and antibody, as well as […]

Determine levels of H3.1-containing, circulating cell-free nucleosomes Active Motif’s Nu.Q™ H3.1 Assay Kit is designed for the detection of levels of Histone H3.1-containing, circulating cell-free nucleosomes (cf-nucleosomes) in human serum in a high-throughput format. Histone H3.1, or the canonical form of Histone H3, is deposited during DNA replication and possibly also during repair. Study of […]

Perform ChIP on FFPE samples Formalin-fixed paraffin-embedded (FFPE) clinical samples are a valuable resource for retrospective research. However, working with FFPE samples presents a challenge for epigenetics techniques such as ChIP. Active Motif has leveraged its epigenetics expertise to develop commercially available kits to enable ChIP from FFPE tissue for use in Next Generation Sequencing […]

Determine total levels of circulating cell-free nucleosomes Active Motif’s Nu.Q™ Total Assay Kit is designed for the detection of global levels of cell-free circulating nucleosomes (cf-nucleosomes) in human serum in a high-throughput format. For added convenience and a more quantitative interpretation of results, the Nu.Q™ Total Assay Kit also includes a recombinant nucleosome protein for […]

One of the biggest challenges associated with preparing high quality ChIP-Seq libraries is sample loss. Traditional library preparation methods require multiple end-repair and ligation steps which contribute to loss of material. To overcome this challenge, Active Motif has introduced its TAM-ChIP technology to perform chromatin immunoprecipitation and next-generation library preparation at the same time. TAM-ChIP […]

Active Motif’s new Low Cell ChIP-Seq Kit offers a complete ChIP-Seq workflow using as few as 1,000 to 50,000 cells per chromatin preparation. This surpasses the limitations of traditional ChIP-Seq protocols which often require more than 100,000 cells for sample preparation. With Low Cell ChIP-Seq, limited sample material, difficult to culture cell lines and small […]

Chromatrap®’s new ChIP-Seq kit utilizes the solid state technology to deliver a streamlined ChIP-seq protocol from small cell numbers and low chromatin concentrations. Specifically adapted for broader chromatin concentrations, Chromatrap® ChIP-seq now combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of […]

The success of a ChIP assay is highly dependent on the quality of chromatin prepared. The sonication shearing kit provides buffers to optimise the shearing conditions of your chromatin by sonication. Isolation of good quality, suitably fragmented chromatin is the most important prerequisite for a successful ChIP assay. Chromatin can be sheared enzymatically or by […]

Ideal for tissue samples, biopsies and difficult to lyse samples.  The sensitivity of the unique solid state platform ensures that small samples can be used with efficient enrichment of both high and low abundant targets. Chromatrap® FFPE ChIP kits provide a quicker, easier and more efficient way of performing ChIP assays from a range of […]

The Tag-ChIP-IT® Kit offers a method to perform chromatin immunoprecipitation (ChIP) on transcription factor sequence variants, mutations and truncations without the need for protein-specific antibodies. Simply create an expression construct containing the protein of interest in-frame with a C-terminal AM-tag. Active Motif’s unique AM-tag sequence is specifically designed to work in ChIP. It has minimal […]

Whether you are performing drug discovery screening, analyzing enzyme kinetics or monitoring changes in histone modifications, substrate selection is critical. Nucleosomes offer the advantage of providing a more biologically relevant substrate when compared to histone proteins alone or synthetic peptides. To best mimic cellular biology with your in vitro assay, use Active Motif’s Recombinant Nucleosomes. […]

Active Motif’s new ChIP-Bisulfite-Sequencing (ChIP-Bis-Seq) Kit offers a highly optimized and user-friendly method to directly interrogate both the chromatin and DNA methylation profile on the same sample within a single experiment. The kit combines a chromatin immunoprecipitation (ChIP) procedure for target-specific enrichment of protein-bound or modified chromatin with bisulfite conversion and sequencing (Bis-Seq) to provide […]

Abcam’s High-Sensitivity ChIP Kit (ab185913) is a complete set of optimized reagents to carry out a successful chromatin immunoprecipitation procedure in a high throughput format starting from mammalian cells or tissues. The highly specific and sensitive kit is suitable for selective enrichment of a chromatin fraction containing specific DNA sequences using various mammalian cell/tissues. The […]

Histone purification system for high throughput applications Active Motif’s Histone Purification Microplate Kit enables purification of core histones (H2A, H2B, H3 and H4) using very small amounts (as little as 0.9 x 105 cells) of starting material, and its microplate spin column format enables elution into a 96-well plate to easily transition into downstream high-throughput […]

Perform ChIP on difficult-to-lyse PBMCs: Active Motif’s ChIP-IT® PBMC Kit overcomes the challenges of generating quality chromatin for use in ChIP from peripheral blood mononuclear cells (PBMCs). PBMCs, including lymphocytes (T cells, B cells and NK cells), monocytes and dendritic cells, constitute a critical component of the peripheral immune system and are often used in […]

Perform ChIP from FFPE samples Formalin-fixed paraffin-embedded (FFPE) clinical samples are a valuable resource for retrospective research. However, working with FFPE samples presents a challenge for techniques such as ChIP. Active Motif has leveraged its ChIP expertise to develop the first commercially available kits to enable ChIP from FFPE tissue for use in Next Generation […]

The HDAC-Glo™ Class IIa Assays are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) Class IIa from cells, extracts or purified enzyme sources. The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a coupled […]

The HDAC-Glo™ 2 Assays are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) Class I enzyme 2 from cells, extracts or purified enzyme sources. The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a […]

The Zymo-Spin™ ChIP Kit from Zymo Research provides a streamlined ChIP procedure for investigating protein-DNA interactions that have been “fixed” in their natural state and can be used to effectively identify binding sites for transcription factors, co-factors, and other DNA regulatory proteins. Features: Unique workflow features a micro-elution (≥6 µl) spin column for purification of […]

Don’t let some big corporation dictate your ChIP protocol! For researchers interested in streamlining their own workflow, Active Motif offers Protein G Agarose Columns. These are ready-to-use ChIP columns that contain the same high affinity protein G agarose beads included in the ChIP-IT® High Sensitivity Kit. The beads have been specifically engineered to eliminate non-specific […]

The combination of ChIP with genome-wide analysis using Next-Generation sequencing (ChIP-Seq) is a powerful approach that can provide insights into gene regulation by identifying global binding sites for proteins of interest. Active Motif’s ChIP-IT® ChIP-Seq Kit provides proven reagents, streamlined protocols and validation controls to give you confidence in successful ChIP-Seq using the Illumina®sequencing platforms. The […]

The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification. This kit includes two different enzymes for nucleosomal DNA preparation: Atlantis dsDNase and micrococcal nuclease. Atlantis dsDNase is a double-strand DNA specific endonuclease […]

The ChIP-IT® High Sensitivity (HS) Kit is designed to give superior performance when using antibodies against low abundance transcription factors, antibodies with sub-optimal binding affinities or when starting with a limited number of cells. Higher signal and lower non-specific DNA pulldown is achieved through the incorporation of specialized protein G agarose beads and optimized fixation, […]

NOMe-Seq is a first of its kind kit to simultaneously analyze nucleosome occupancy and CpG methylation status on the same DNA strand. NOMe-Seq (Nucleosome Occupancy and Methylome sequencing) is a published method that was developed by the Peter Jones lab at USC and has been validated for gene-specific analysis using both traditional cloning and Sanger […]

Benchtop shearing for NGS and ChIP M220 Focused-ultrasonicator is designed for Next-Gen Sequencing and ChIP applications. The compact size and ease-of-use of the M220 Focused-ultrasonicator make it the ideal Chromatin shearing solution for any lab.  Compatibility with the truChIP™ Low Cell Chromatin Shearing kits provides sensitive ChIP assays with fewer than 1 million cells. Integrated […]

Novus’ ChromataChIP™ Kits are a complete ChIP solution to help you perform chromatin immunoprecipitation efficiently and accurately in your laboratory. We’ve taken all the guesswork out of ChIP by testing multiple component options to make the best kit on the market. Simple to use – ChromataChIP™ is the simplest kit on the market. Our straightforward […]

Epi-Plus™ antibodies are the most well validated antibodies available for epigenetics research. All Epi-Plus™ antibodies are multi-assay validated, including testing by dot blots using modified peptide arrays, western blots, and chromatin immunoprecipitation (ChIP). Novus Biologicals and 21st Century Biochemicals have collaborated to produce this superior line of Epi-Plus™ antibodies.

The AbSurance™ Complete Core Histone Antibody Specificity Array is comprised of 89 high quality modified and unmodified histone peptides ( >95 % purity) spotted on Immobilon® P PVDF membranes in a 96-well format. One membrane contains 46 histone H3 peptides (8 acetylated, 27 methylated, 5 phosphorylated, and 6 unmodified). The other membrane contains 43 histone […]

The traditional ChIP methods, based on agarose or magnetic beads, involve many steps of separation, pipetting and re-suspension. These sequences can either be done by magnetism or centrifugation, which are repetitive and prone to errors. Chromatrap® is a new technology that offers a quicker, easier and more efficient way of undertaking chromatin immunoprecipitation assays. These kits […]

The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument. Each kit includes a complete set of validated, quality controlled reagents, positive and negative […]

The Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument.Each kit includes a complete set of validated, quality controlled reagents, plus a detailed protocol. […]

The SIRTainty™ Class III HDAC Assay utilizes a novel patent pending technology for the sensitive detection of all known sirtuin family members. This easy to perform assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a substrate providing a direct assessment of the activity of class III HDAC […]

Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome […]

Perform automated ChIP (Chromatin Immunoprecipitation[application ChIP]) from as low as 1000 cells. Ideal for Stem cells, embryos and biopsies. The Diagenode’s Auto ChIP kit is designed to perform automated immunoprecipitation of Chromatin using the SX-8G IP-StarTM Automated System enabling highly reproducible results and allowing for high throughput. Use from 1,000 – 100,000 cells per IP […]

Perform Automated MeDIP (Methylated DNA Immunoprecipitation) to estimate DNA methylation status of your sample using the 5-methylcytidine antibody. Diagenode’s Auto MeDIP kit has been optimized on the SX-8G IP-StarTM Automated System enabling highly reproducible results and allowing for high throughput assays. Generate highly consistent results with internal controls in 24h Minimize error with many reagents […]

The Bioruptor® uses a unique system to uniformly process multiple samples in sealed tubes of 0.5ml to 50ml capacity. An excellent device for shearing chromatin or DNA, cell lysis and many other applications: Chromatin Shearing DNA Shearing cell Lysis Disruption Cleaning Disaggregation Extraction Dissolution Emulsification Homogenization Comminution Sonochemistry Dispersion Mixing Synthesis Catalysis Degassing Microencapsulation Hydrolyzation […]

  FFPE samples are highly valuable for their ability to relate clinical outcomes to disease states and epigenetic profiles. Active Motif’sFFPE Bisulfite Conversion Kit is designed for DNA methylation analysis of formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues to provide a DNA methylation profile at single-base-pair resolution.   Schematic of bisulfite conversion. Since FFPE samples are […]

Active Motif’s Global 5-hmC Quantification Kit is an ELISA-based assay designed to detect and quantify 5-hydroxymethylcytosine (5-hmC) levels in genomic DNA from different sample types (e.g. normal and diseased), treatment conditions, clinical outcomes or environmental backgrounds. The Global 5-hmC Quantification Kit enables higher throughput processing of samples, requires only nanogram amounts of genomic DNA per […]

Global DNA Methylation Assay to quantify genome-wide 5-mC levels in human DNA Simple ELISA assay in a flexible strip-well microplate format Detects as low as 0.5% methylation Detects methylation from as little as 10 ng DNA/well LINE-1 probe used for DNA capture for better specificity Biologically relevant standards provided for improved quantification Active Motif’s Global […]

EpiSeeker 5-formylcytosine DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify 5-formylcytosine (5-fC) in a microplate-based format. It is suitable for use with DNA isolated from any species (mammals, plants, fungi, bacteria, viruses, etc.) in a variety of forms including, but not limited to, cultured cells, fresh and frozen […]

The MethylEdge™ Bisulfite Conversion System provides a rapid, efficient method to perform bisulfite conversion with minimal DNA fragmentation in less than two hours. The rapid protocol and complete conversion mean that you can produce completely converted DNA ready for your choice downstream assays with minimal preparation and hands-on time. Coupled with purification chemistries and flexible, robust amplification […]

SureSelectXT Human Methyl-Seq is the first comprehensive target enrichment system to enable researchers to focus on the regions where methylation is known to impact gene regulation: CpG islands, CpG island shores, undermethylated regions, promoters, and differentially methylated regions (DMRs). SureSelectXT Human Methyl-Seq Uniquely delivers more information than methylation microarrays by detecting individual CpGs Increases throughput […]

The ability to efficiently detect and quantify DNA methylation (i.e., 5-methylcytosine) has become essential for epigenetic-based research.  To date, a number of methods have been developed for this purpose including high-performance capillary electrophoresis, bisulfite sequencing, and methylated DNA immunoprecipitation. The 5-mC DNA ELISA Kit is a convenient and powerful tool that allows the researcher to […]

The Quest 5-hmC™ DNA ELISA Kit is both sensitive and specific and can be used to accurately detect 5-hmC DNA in a variety of samples. The kit is compatible with a wide range of input DNA including intact vertebrate, plant, and microbial genomic DNA, as well as enzyme-digested and mechanically sheared fragments. The Control DNA […]

Specific glycosylation of 5-hydroxymethylcytosine (5-hmC) 5-hmC Glucosyltransferase catalyzes the transfer of a glucose moiety from uridine diphosphoglucose (UDPG) to existing 5-hmC in DNA to form glucosyl-5-hydroxymethylcytosine (glucosyl-5-hmC). 5-hmC Glucosyltransferase does not modify cytosines or methylated cytosines (5-mC). Glucosylation of 5-hmC can be used for sequence-specific, locus-specific, or global quantification of 5-hmC. Validation: 5-hmC Glucosyltransferase was […]

CpGenome™ Human Methylated & Non-methylated DNA Standards are purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. The CpGenome™ Human Methylated DNA Standard has been methylated enzymatically at all CpG dinucleotides by M. SssI methyltransferase 2. These standards are […]

CpGenome™ 5-mC and 5-hmC DNA Standard Set contains three linear, double-stranded DNA (897 bp) with the same sequence, but each contains 100 % 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines. This set can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications. Materials Provided: Three vials, each containing 2 µg (40 µL) […]

The EZ DNA Methylation-Lightning™ Kit features rapid and reliable bisulfite treatment and conversion of DNA for methylation analysis. Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is necessary, simply add this unique reagent to a DNA sample, wait about an hour, and let the reaction proceed to completion. DNA denaturation […]

Active Motif’s MethylCollector™ Ultra offers a fast magnetic assay capable of efficiently isolating methylated CpG islands from fragmented genomic DNA. MethylCollector Ultra is based on the Methylated CpG Island Recovery Assay (MIRA) which uses a MBD2b/MBD3L1 protein complex for improved enrichment of CpG dinucleotides. Enriched DNA can be used in many downstream applications, such as […]

The Hydroxymethyl Collector™ Kit is designed to detect and capture DNA fragments containing 5-hydroxymethylcytosine (5-hmC) DNA methylation. The Hydroxymethyl Collector kit utilizes a β-glucosyltransferase enzyme to transfer a modified glucose moiety to 5-hydroxymethylcytosine residues in double-stranded DNA. This modified glucose is then used to chemically attach a biotin conjugate for capture and enrichment with streptavidin […]

5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC […]

5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC […]

Epigenetic modifications are regarded as fundamental to the regulation of gene expression. DNA methylation is one such modification that plays crucial roles in widespread biological phenomena including the regulation of gene activity, gene imprinting, embryonic development and X-chromosome inactivation in higher organisms. Current methods used to evaluate DNA methylation including bisulfite sequencing, Methylation Specific PCR […]

The Quest 5-hmC Detection Kit™ from Zymo Research allows for sequence specific detection of 5-hydroxymethylcytosine within DNA using a simple and efficient reaction setup. Utilizing a robust and highly specific 5-hmC Glucosyltransferase enzyme, 5-hydroxymethylcytosine in DNA is specifically tagged with a glucose moiety yielding a modified base, glucosyl-5-hydroxymethylcytosine. Product Highlights: Method to specifically distinguish 5-hydroxymethylcytosine […]

Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is […]

Active Motif’s RNA Subcellular Isolation Kit is designed to efficiently isolate separate nuclear and cytoplasmic fractions of RNA for downstream analysis. This method can be used to isolate both long and short RNA sequences from cells or tissue without cross-contamination or the use of phenolic compounds. RNA molecules exist at different levels of maturation and […]

Long noncoding RNAs (lncRNAs) have been shown to play important roles in the regulation of both transcription and chromatin organization. But, the promoters of lncRNA genes appear to be regulated differently than those of protein-coding genes. The LightSwitch Luciferase Assay System makes it fast & easy to study lncRNA promoter regulation using the same materials […]

The EZ RNA Methylation™ Kit features rapid and reliable bisulfite treatment and conversion of cytosines in RNA for methylation analysis. The kit streamlines the three-step process for complete conversion of cytosine in into uracil. RNA denaturation and bisulfite conversion processes are combined into a single step. No buffer preparation is necessary. The RNA Conversion Reagent […]

The LightSwitch™ Luciferase Assay System includes over 30,000 regulatory elements from the human genome that have been cloned into LightSwitch reporter vectors. With over 18,000 human promoters and 12,000 3´UTRs available as transfection-ready LightSwitch luciferase reporter vectors, you can perform reporter assay experiments immediately. The LightSwitch System makes it easy for you to measure the […]