Marligen’s Vantage microRNA Labeling Kit utilizes a proprietary labeling technology to provide exceptional sensitivity for the detection of endogenous microRNAs. This method can label any RNA sample including total RNA, enriched low molecular weight (LMW) RNA, amplified senseRNA and degraded RNA. The simple protocol consists of just two steps. The 3’end of the RNA is poly(A) tailed followed by the ligation of biotinylated 3DNA dendrimers containing 15 biotins to provide signal amplification.
Advantages:
- Labeling can be completed in 45 minutes
- Highly sensitive labeling method requires little input RNA (as little as 100g total RNA extracted from tissues)
- LMW RNA enrichment not necessary
- Labels degraded RNA from FFPE samples
- Biotinylated RNA suitable for multiple applications
- Compatible for use with xMAP microRNA profiling kits