Let’s say you want to get the ball rolling for studies on a new disease. You know you want to search for any important changes in methylation patterns; but with a whole genome worth of ground to cover, how do you decide where to look first? Active Motif now offers the MethylPath™ MeDIP-Seq Service that can put those answers at your fingertips faster than TMZ can jump on a scandalous Tweet.
The genome-wide power of Next-Gen sequencing has significantly broadened research into epigenetic events, but at the same time antibody selection, sample prep and data analysis are often enough of a hassle that it isn’t in the experimental bag-of-tricks for many labs. Active Motif Epigenetic Services now makes these cutting-edge techniques accessible to more scientists in the trenches than ever.
MethylPath™ MeDIP-Seq Service will get you where you need to go.
Get the MeDIP Advantage
MeDIP-Seq is a great way to survey DNA methylation anywhere in the genome. A super specific antibody for 5-methylcytosine is used to grab methylated genomic DNA fragments, which are then prepped for sequencing. Alignment of the various genomic bits with a reference genome then signals where DNA methylation is found, giving you a snapshot that you can use to look for biomarkers or regions you want to take a closer look at. Unlike RRBS which only scans about 10% of CpGs, MeDIP-Seq, can cover regions all across the genome. Plus, assay results closely mirror CpG densities, increasing the ability to find differentially methylated regions (DMRs).
Active Motif’s experts designed their MethylPath™ MeDIP-Seq service as a complete, end-to-end, genome-wide way to identify DMRs, so that all you have to do is supply the samples, and they’ll handle the rest. Once you submit purified DNA, frozen tissues or cell pellets, Active Motif scientists spring into action to:
- Prepare the sample
- Perform MeDIP with 5-mC mAb
- Amplify the enriched DNA sample
- Perform Next-Gen sequencing
- Analyze the data
All you have to do is figure out which high-end journal your next paper ought to be featured in. A quick email to the Active Motif Epigenetic Services team will get you started. And while you’re at it, see if they can also help you out with: