Over the last decade, the ChIP process has seen more enhancements than the Orange County Housewives. Improved antibodies, beads, and fine-tuned buffers—they’ve all ChIP’d in to take this useful method primetime in labs all over the globe. But there’s always room for improvement.
Turning Up the Pull Down for Small Sample ChIP
Some of the most interesting epigenetic action is hiding in smaller, homogenous samples. The Chromatrap® Pro-A ChIP Kits use spin columns packed with protein A discs comprising a top secret internal structure that soak up chromatin complexes like a sponge. It’s these über -efficient columns that boost DNA enrichment and signal to noise ratios in the assay. How much? The Porvair team and their collaborators routinely see:
- DNA pull down up to 25 times higher than with bead based procedures
- DNA enrichment levels that boost signal to noise ratios 2 to 3 times better than competing procedures
- Solid performance in as few as 15,000 cells per assay
Just think how many bivalent domains you can cram in there
Smoothing Out ChIP
In many cases you have to earn better performance, but Chromatrap® Pro-A ChIP Kits actually make the ChIP process simpler as well. How’s that possible you ask? It all comes back to the discs.
- The chemical reactions take place inside the disc, eliminating suspension or re-suspension by pipette – in other words there are no more beads to fiddle with
- Negligible background binding means no pre-blocking steps
- The discs are easily and efficiently flushed clear of unwanted chromatin
See it in Action
The Chromatrap® technology is new, but many labs are already putting these kits to work. Check out some of the case studies or download their App note over at: chromatrap.com