Bisulfite conversion kits for DNA methylation studies are notorious for causing high levels of sample DNA fragmentation. What a pain. You may as well be flushing a portion of your hard-won samples down the sink. And the only way around it is to start off with more sample in the first place, which seriously limits the type of sample types you can work with.
Unless you are using Promega’s MethylEdge™ Bisulfite Conversion System that is.
Say “No” to Fragmentation
The MethylEdge™ Bisulfite Conversion System has been specifically configured to perform bisulfite conversion of >99% of the DNA in your samples, with minimal sample DNA fragmentation. Check out the gel on the left for a comparison of the MethylEdge™ system with other bisulfite kits on the market. Hint: the two high molecular weight smears in P1 and P2 are from the MethylEdge™ system preps.
We’re usually not that excited by gel images, but just look at those lines
Ditch the Cell Lines
Nothing against, PC3, CHO, and Hela; hey we’ve never had a book written about our beginnings, but there’s only so much big samples can tell you. Less fragmentation means that you can start with less DNA , opening the door to a wider range of sample inputs that often have more biological relevance.
The MethylEdge™ Bisulfite Conversion System can process anything from 100pg-2µg of DNA, making studying DNA methylation in samples like FFPE or biopsies possible.
Added Performance, Not Steps
Usually, improved performance comes with a price; like a bazillion hours of hands on time, but the MethylEdge™ Bisulfite Conversion System has been designed with your need for speed and convenience in mind.
All reagents in the kit are ready to go straight out of the box, and are stable at room temperature. So there’s no freeze-thawing, mixing or re-suspending to slow you down. Just add ethanol to the wash buffer, and off you go.
- The protocol takes just 2 hours. No arduous steps, no over-night incubations.
- 100 pg to 2 ug of DNA to >99% bisulfite conversion within 2 hours, with minimal fragmentation. We were impressed too.
Get Your Edge Back
For more information on Promega’s MethylEdge™ Bisulfite Conversion System, check out its product information page. Coupled with robust upstream purification and quantitation technologies and effective downstream amplification solutions, the MethylEdge™ Bisulfite Conversion System offers convenience, performance, a whole lot more intact DNA for your DNA methylation research.