It’s never a bad idea to insure the important things in life against disaster. Wouldn’t it be great if epigenetics researchers could do the same for an upcoming experiment? Although they aren’t exactly in the insurance game, EMD Millipore has designed the new AbSurance™ Histone Antibody Specificity Arrays to act as a protective policy against the damage caused by non-specific histone antibodies.
Get Your Histone Antibodies Covered
When scoping out various histone modifications, especially closely related ones, it’s critical to have highly specific antibodies; but as we know, not every antibody goes through strenuous characterization. AbSurance™ Histone Peptide Arrays let you quickly and easily screen histone antibodies yourself to find any potential cross-reactivity problems before they pop up to bite you later – like after a pricey ChIP-seq run gone wrong, or when a reviewer holds up your latest publication over a questionable antibody.
AbSuranceTM is one policy that always pays off.
AbSurance™ Histone Peptide Arrays use a familiar Western-blot style procedure that’s easy for any lab to adopt. Here are some more features that AbSurance™ Arrays are sporting:
- Peptide arrays are printed on easy-to-handle PVDF membranes designed with landmarks for easy spot identification including a cut corner at position A1 and positive control IgG spots. Plus, each peptide spot is marked with inert dyes to show location and an alignment grid and peptide map comes with the manual.
- Detection is done with chemiluminescense on film or CCD imagers, so no special software or analysis is necessary.
- Includes positive control primary antibodies from rat, mouse, sheep, and rabbit
- High quality peptides (over 95% pure) are verified by mass spectrometry, and then printed in two concentrations (10ng and 100ng) in order to fish out both strong and weak cross-reactivity
- Proprietary production process delivers consistent and uniform spotting of peptides making quantification possible.
How to Become an AbSurance™ Beneficiary
It’s easy to cash in on an AbSurance™ policy. Just head to the EMD Millipore website and check out all three versions:
- AbSurance™ Histone H2A, H2B, H4 Antibody Specificity Array has 43 histone H2A, H2B and H4 peptides (13 acetylated, 16 methylated, 6 phosphorylated, and 8 unmodified).
- AbSurance™ Histone H3 Antibody Specificity Array contains 46 histone H3 peptides (8 acetylated, 27 methylated, 5 phosphorylated, and 6 unmodified).
- AbSurance™ Complete Core Histone Antibody Specificity Array comes with total of 89 histone H3, or H2A, H2B, and H4 peptides on two membranes.
Get them as individual H3 or H2A, H2B, H4 Arrays, or as a complete set for easy screening.