New England Biolabs is hosting a free webinar titled:
Global and locus specific 5-hydroxymethylcytosine detection and quantification
Tues. March, 27th 2012 at 9am PDT
It will discuss simple and quick enzymatic methods for global, as well as sequence-specific, determination of both DNA modifications throughout various genomes. T4 phage B-glucosyltransferase has emerged as the enzyme of choice for genome-wide 5-hmC quantifications. Similarly, using MspI sensitivity to glucosylated 5-hydroxymethylcytosine, NEB has established a scheme to interrogate differential methylation status at specific loci. In this webinar, various enzymatic techniques and their advantages will be discussed.