In this webinar, Dr. Xi Chen takes publicly available real ChIP-seq data sets to show you how to perform ChIP-seq routine analysis using a combination of different online tools available. This provides an opportunity for bioinformatics beginners and wet-lab biologists who do not have much experience with unix operating systems to have an idea about what ChIP-seq data sets look like and what kind of results one can extract from ChIP-seq experiments.
- QC of sequencing reads
- Read alignment: mapping sequencing reads to the reference genome
- Peak calling: identify binding sites of the protein of interest
- Data visualisation
- De novo motif discovery, gene ontology of binding sites and heatmap generation