In the past, the route to locus-specific methylation detection resembled more of a brutal molecular biology pentathlon than a microfuge tube. If you’re into that kind of abuse, that’s cool, but you probably won’t be interested in Zymo’s new OneStep qMethyl™ Kit. This slick system closes the lid on the old school tool set and brings bisulfite-free, high-throughput, quantitative DNA methylation analysis to your bench.
You’ll find no bisulfite in this protocol, unless your lab mate spilled a little bit on it (good thing she brings bagels on Thursdays). At the heart of OneStep qMethyl™ Kit are procedures that you’re probably familiar with, methyl sensitive restriction digestion, qPCR, and a whole lot of optimization (compliments of Zymo of course). As the name implies, the whole process has been streamlined down to a single step that takes place in one tube…just add DNA sample and primers to the reaction mix, fire up the qPCR machine and you’re ready to go.
DNAm Analysis OneSteps Up
Don’t be fooled by the simplified OneStep qMethyl™ protocol. The kit has the flexibility of a yoga master, enabling you to:
- Study one locus in a bunch of samples, or
- Screen several loci in a single sample
This makes the OneStep qMethyl™ approach a super convenient tool for hungry researchers validating DNAm results from massive sequencing runs or for those deep in biomarker validation mode that need a high-throughput method for screening biological samples quickly and efficiently.
OneStep Up Today
Work smarter, not harder and take the next step in your DNAm analysis. Check out the OneStep qMethyl™ Kit at the Zymo Research today.