THE DIRECT-ZOL™ RNA MINIPREP provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI-Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure, RNA-Bee or similar acid-guanidinium-phenol based reagents. Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product.
The procedure is easy: simply apply a sample in TRI-Reagent® to the spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. Small RNAs (=>17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
What’s included in the kit?
• TRI-Reagent® (with select kits)
• Direct-zol™ RNA PreWash (concentrate)
• RNA Wash Buffer (concentrate)
• DNase/RNase-Free Water
• Zymo-Spin™ IIC Columns
• Collection Tubes
• Instruction Manual