Roche NimbleGen offers six types of promoter designs to suit your DNA Methylation analyses:
- 2.1M Deluxe Promoters are single array designs for human and mouse that cover ~10 kb of all annotated promoters. In addition, these arrays tile through all annotated CpG islands, all annotated miRNA promoters, and manually selected ENCODE regions (human only). All regions are tiled at 100bp probe spacing. These arrays also include new positive, negative and non-CpG control regions to facilitate assessment of array and sample performance.
- 3x720K CpG Island Plus RefSeq Promoter Arrays are multiplex slides with 3 identical arrays per slide enabling the simultaneous analysis of 3 DNA Methylation samples for increased sample throughput. These arrays cover the annotated CpG islands as well as the promoters of the well characterized RefSeq genes derived from the UCSC RefFlat files and are available for human, mouse and rat. These arrays also include new positive, negative and non-CpG control regions to help facilitate assessment of array and sample performance.
- 385K Two-Array Sets contain all annotated splice variants and alternative transcription start sites. This array set provides the most comprehensive tool for genome-wide mapping of transcriptional regulatory elements including all RefSeq genes, the Mammalian Gene Collection, and UCSC known genes to encompass the most comprehensive list of transcripts available. Designs are available for human, mouse, rat, and A. thaliana.
- 385K RefSeq Promoters are single array designs containing all known well-characterized RefSeq genes (NM prefix). The promoter regions on these arrays are covered by 50-75mer probes with approximately 100bp spacing, dependent on the sequence composition of the region. Designs are available for human, mouse andrat.
- 385K RefSeq XM Promoters are single array designs containing model reference sequences produced by NCBI’s Genome Annotation Project (those accessions beginning with XM) that are predicted by genome sequence analysis. The entries represent ab initio predictions, or have some level of transcript or homology to known genes to support the gene predictions. They represent the transcripts and proteins that are annotated on the NCBI Contigs, and they may be different from GenBank submissions for mRNAs and/or the curated RefSeq records with NM, NR, NP accession prefixes. Designs are available for human and mouse.
- 385K Minimal Promoters are currently available for A. thaliana.