Product Overview: DNA methylation is a covalent modification of the cytosine ring at the 5′ position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5′-CpG-3’dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylatin plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.
Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
EpiSeeker DNA purification & modification Kit (ab117124) allows the user to isolate and modify DNA efficiently. The entire procedure can be completed within 2 hours and 20 minutes is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
View EpiSeeker DNA purification & modification Kit on Abcam’s website