Product Overview: Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of the proper higher order structure of DNA and is consequently essential for chromosome condensation. It is also involved in gene silencing.
EpiSeeker Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric) (ab115094) enables the user to measure global mono-methylation of histone H4-K20. The global H4-K20 mono-methylation can also be changed by inhibition or activation of HMTs, making quantitative detection of global mono-methyl histone H4-K20 useful in developing a better understanding of epigenetic regulation of gene activation/repression and for developing HMT-targeted drugs. ab115094 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
View EpiSeeker Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric) on Abcam’s website