High throughput (96-well), complete bisulfite conversion of DNA directly from blood, tissue, or cells. Compatible with small sample inputs – as few as 10 cells or 50 pg DNA. Well-suited for FFPE and LCM-derived samples. The 96-well format of EZ-96 DNA Methylation-Direct Kit is a further refinement of our popular 96-well EZ DNA Methylation and EZ DNA Methylation-Gold Kits.
The EZ DNA Methylation-Direct Kit features simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this kit makes it possible to amplify bisulfite converted DNA from as few as 10 cells or 50 pg DNA.
Like the EZ DNA Methylation-Gold Kit, DNA denaturation and bisulfite conversion processes are combined into a single step. All kits streamline the three step process of bisulfite conversion of non-methylated cytosine in DNA into uracil. In addition, the methylation kits share innovative in-column desulphonation technology that eliminates otherwise cumbersome DNA precipitation steps while ensuring researchers consistent results every time. The kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
View EZ-96 DNA Methlyation Direct™ Kit -Deep Well Plates on Zymo Research’s website