The EZ Bisulfite DNA Clean-up Kit™ has been specifically designed for the purification of bisulfite-treated DNA from any “homebrew” or commercial reaction mixture containing bisulfite. This product features innovative Fast-Spin, in-column desulfonation and wash technologies that eliminate DNA loss, buffer carryover, and the need for ethanol/isopropanol precipitations. The procedures are easy and DNA clean-up can be completed in just minutes. Bisulfite-treated DNA purified with this product is ideal for PCR amplification for downstream DNA methylation analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
Features include:
- Consistent, high yield recovery of bisulfite-treated DNA from any homebrew or commercial reaction mixture containing bisulfite.
- Simple spin column procedure with small elution volumes (more than 10 µl) for concentrated DNA.
- Recovered DNA is ideal for downstream methylation analysis including PCR, endonuclease digestion, sequencing, arrays, etc.
- DNA Input: Up to 5 µg DNA (per column) from homebrew or commercial reaction mixtures containing bisulfite.
- DNA Recovery: Typical yields are between 80-90%.
- Elution: DNA can be eluted with more than 10 µl M-Elution Buffer or water.
- 96 Shallow & Deep Well versions available