The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation Kit. The EZ DNA Methylation-Gold Kit integrates the DNA denaturation and bisulfite conversion processes into one step. This is accomplished using temperature denaturation to replace chemical denaturation with sodium hydroxide in the previous protocol. Also, the kit has been streamlined for high yield recovery of DNA following bisulfite treatment. Both kits are based on a three step reaction process between cytosine and sodium bisulfite resulting in cytosine being converted into uracil. The EZ DNA Methylation-Gold and EZ DNA Methylation Kits share innovative in-column desulphonation technology that eliminates cumbersome DNA precipitation steps while providing researchers consistent results every time. The kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
Features include:
- Complete bisulfite conversion of GC-rich DNA in less than 3 hours.
- A coupled DNA denaturation/bisulfite treatment step streamlines the conversion of unmethylated cytosines into uracil.
- DNA precipitations are omitted. Instead, DNA is cleaned and desulphonated in a single step using state-of-the-art spin columns.
- Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
- Recovery Volume: Purified, bisulfite treated DNA is recovered in more than 10 µl provided elution buffer.
- Input DNA: 500 pg – 2 µg DNA per treatment with 200 – 500ng being optimal.
- Sample Sources: Plasmid, genomic, and endonuclease digested DNAs.
- 96 Shallow & Deep Well versions available