The EZ DNA Methylation Kit uses a simplified procedure and streamlines the bisulfite method for DNA methylation analysis. The kit is based on the three step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The EZ DNA Methylation Kit’s innovative in-column desulphonation reaction eliminates several precipitation steps and provides researchers with consistent reaction conditions. The kit is designed to reduce template degradation, minimize DNA loss during treatment and clean-up, and result in the complete conversion of unmethylated cytosines. The recovered, treated DNA template is ideal for Methylation Specific PCR (MSP) followed by methylation analysis using restriction endonucleases, sequencing, microarrays, etc.
Features Include:
- Streamlined, proven procedure for bisulfite conversion of DNA.
- Desulphonation and recovery of bisulfite-treated DNA with a spin column.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
- Format: Chemical denaturation of DNA followed by sodium bisulfite modification, Spin Column.
- Recovery Volume: Purified, bisulfite treated DNA is recovered in more than 10 µl of provided elution buffer.
- Input DNA: 500 pg – 2 µg DNA per treatment with 200 – 500ng being optimal.
- Sample Sources: Plasmid, genomic, and endonuclease digested DNAs.
- 96 Shallow & Deep Well versions available